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    Plasmid DNA Purification Kit (Solution Based)

    Plasmid DNA Purification Kit (Solution Based)

    Isolate Plasmid DNA for Every Application

    • Preferred method for plasmid DNA extraction from bacteria ever since
    • A simple 5 step protocol
    • Convenient and cost effective for Teaching Practice
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    The isolation of plasmid DNA from bacteria is a crucial technique in molecular biology and is an essential step in many procedures such as cloning, DNA sequencing, transfection, and gene therapy. These manipulations require the isolation of high purity plasmid DNA. The purified plasmid DNA can be used for immediate use in all molecular biology procedures such as digestion with restriction enzymes, cloning, PCR, transfection, in vitro translation, blotting and sequencing.

    Alkaline lysis is a method used in molecular biology, to isolate plasmid DNA or other cell components such as proteins by breaking the cells open. Bacteria containing the plasmid of interest is first grown, and then allowed to lyse with an alkaline lysis buffer consisting of a detergent sodium dodecyl sulfate (SDS) and a strong base sodium hydroxide. The detergent cleaves the phospholipid bilayer of membrane and the alkali denatures the proteins which are involved in maintaining the structure of the cell membrane. Through a series of steps involving agitation, precipitation, centrifugation, and the removal of supernatant, cellular debris is removed and the plasmid is isolated and purified.

    Applications

    • PCR
    • Sequencing
    • Cloning
    • In vitro transcription
    • Restriction enzyme digests
    • Random primed labeling
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    Format Manual; Solution Based (Scalable)
    Preparation time 40 minutes
    Equipment required Microcentrifuge
    Minimum elution buffer volume 50  µl
    Culture volume for high-copy plasmids 1–5 ml
    Culture volume for low-copy plasmids/cosmids 1–10 ml

    Yield is variable and depends both on the particular E. coli strain used and the cell density of the bacterial culture.

    Kit Content

    Contents

    Storage  Temperature

    (upon receipt)

    Buffer P1

    15-25°C

    Buffer P2

    15-25°C

    Buffer P3

    15-25°C

    Storage Conditions and Product Stability

    All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.

    For Research Use Only. Not for use in diagnostic procedures.

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    Items Cat. # Size
    Plasmid DNA Purification kit (Solution Based) SCMR002 50 Prep.
    Plasmid DNA Purification kit (Solution Based) SCMR002A 100 Prep.
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    Plasmid DNA Purification Kit (Solution Based)

    Isolate Plasmid DNA for Every Application

    • Preferred method for plasmid DNA extraction from bacteria ever since
    • A simple 5 step protocol
    • Convenient and cost effective for Teaching Practice
    Edit Content
    Click on the Edit Content button to edit/add the content.
    Edit Content
    Click on the Edit Content button to edit/add the content.
    Edit Content
    Click on the Edit Content button to edit/add the content.
    Edit Content
    Items Cat. # Size
    Plasmid DNA Purification kit (Solution Based) SCMR002 50 Prep.
    Plasmid DNA Purification kit (Solution Based) SCMR002A 100 Prep.