gDNA Extraction Kit (Based on Organic Extraction)

[for Experienced user]

Purify total genomic DNA with the Good quality and quantity

Easily scalable
Efficiant for difficult to lyse samples
Can be used for wide variety of starting Material.
High yield and high quality DNA ready for any application
Based on Phenol/chloroform/isoamyl alcohol (PCI) extraction & alcohol precipitation

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This kit provides a rapid method for the isolation and purification of total DNA from a wide range of plant and fungal species. Total DNA, including genomic DNA, mitochondrial DNA and chloroplast DNA can be purified from fresh or frozen plant tissues, plant cells or fungi samples using this kit. Purified DNA samples can be used for the detection of viral pathogens, as viral DNA is isolated with the plant/fungi DNA. This Kits provide fast and easy silica-based DNA isolation from plant samples in spin column format. Typical yields are 3–200 μg of high-quality DNA, depending on the samples used (e.g., wheat, maize, Arabidopsis, tomato, tobacco) and binding capacity of the silica membrane.

The purified DNA is of the highest integrity, and can be used in a number of downstream applications including PCR, qPCR, SNP, Southern blotting and sequencing.

Applications

This Kit provide purification of ready-to-use DNA from plant samples, including plant cells, plant tissues and fungi.

For Research Use Only. Not for use in diagnostic procedures.

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Features	Specifications
Elution volume	Up to 500 µl (Depends on sample amount)
Time per run or per prep	<1 hour
Sample type	Blood, Bacterial, Tissue
Format	Organic Extraction
Maximum Blood Input	200 µL
Average Yield	Up to 30 µg *
Processing	Manual

*Yield will vary depending on the type and Quantity of sample processed

Storage Conditions and Product Stability

Phenol:Chloroform:Isoamylalcohol solution and Chloroform:Isoamylalcohol should be store at 2°C to 8°C. Remaining solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.

Cautions

This kit is design for Experienced Users only who have skills in Extracting Nucleic acid with Hazardous solutions.

A phenol/chloroform reagent widely used in DNA purification is notoriously hazardous. Phenol is a very strong acid that causes severe burns.

Chloroform is a carcinogen. Exposure to alcohol may increase toxic effects.

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Items	Cat. #	Size
gDNA Extraction Kit (Phenol:Chloroform basaed)	SCMR001	25 Preps.
gDNA Extraction Kit (Phenol:Chloroform basaed)	SCMR001A	50 Preps.

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