For Transformation and Expression of GFP (green fluorescent protein) gene
SLS’ Bacterial Transformation Teaching kit enables students to prepare competent bacterial cells and transform them with a plasmid containing Green Fluorescent Protein.
SLS’ Bacterial Transformation Teaching kit offer engaging and unforgettable lab activities in which students engineer bacteria to express the Green Fluorescent Protein (GFP) and glow green under UV light. These kits enable hands-on learning about the central dogma, gene expression and regulation, genetic engineering, and the biomanufacturing process.
GFP cloning kit enables students to learn the process of gene cloning and the clone confirmation is done by the phenotypic expression of green fluorescent protein (GFP) in E.coli.
Hands-on genetic engineering — from handling plasmids to performing heat shock and plating the transformants, students perform the same transformation protocol used by scientists on a daily basis around the world
Visible, dramatic phenotype — bacteria transformed with GFP Clone glow a brilliant fluorescent green under UV light. Once students transform bacteria with GFP Clone, they never forget the central dogma of molecular biology: DNA > RNA > Protein > Trait — Green Fluorescence
Gene expression and gene regulation — bacteria transformed with GFP show green fluorescence only when they are grown in the presence of Antibiotic. Use this experiment to illustrate that not all genes are expressed at the same time and that environmental factors control when gene expression takes place
Materials Provided in Kit |
Storage upon Receipt |
Positive Control Plasmid (pUC18-GFP) |
-20°C |
E.coli (Host Cell) |
-20°C |
Ampicillin(100mg/ml) |
-20°C |
0.1M Calcium chloride |
2°C – 8°C |
Luria Bertani(LB)Broth |
RT |
Agar |
RT |
All solutions should be kept tightly sealed and stored at above define temperature. These reagents should remain stable for at least 1 year in their unopened containers.
Items | Cat. # | Size |
---|---|---|
Bacterial Transformation Kit (GFP Based) | SCMT010 | 20 Preps. |
For Transformation and Expression of GFP (green fluorescent protein) gene
Items | Cat. # | Size |
---|---|---|
Bacterial Transformation Kit (GFP Based) | SCMT010 | 20 Preps. |
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