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    Bacterial Transformation Kit (GFP Based)

    Bacterial Transformation Kit (GFP Based)

    For Transformation and Expression of GFP (green fluorescent protein) gene

    Features
    • Rapid Transformation of Ready to use Clone with GFP Gene
    • No need of cooling centrifuge
    • User friendly protocol for competent cell preparation and transformation
    • Easy screening of transformed cells by phenotypic expression of Green Fluorescent Protein
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    SLS’ Bacterial Transformation  Teaching  kit  enables  students  to prepare competent bacterial cells and transform them with a plasmid containing Green Fluorescent Protein.

    SLS’ Bacterial Transformation  Teaching  kit   offer engaging and unforgettable lab activities in which students engineer bacteria to express the Green Fluorescent Protein (GFP) and glow green under UV light. These kits enable hands-on learning about the central dogma, gene expression and regulation, genetic engineering, and the biomanufacturing process.

    GFP cloning kit enables students to learn the process of gene cloning and the clone confirmation is done by the phenotypic expression of green fluorescent protein (GFP) in E.coli.

    Features and Benefits

    Hands-on genetic engineering — from handling plasmids to performing heat shock and plating the transformants, students perform the same transformation protocol used by scientists on a daily basis around the world
    Visible, dramatic phenotype — bacteria transformed with GFP Clone glow a brilliant fluorescent green under UV light. Once students transform bacteria with GFP Clone, they never forget the central dogma of molecular biology: DNA > RNA > Protein > Trait — Green Fluorescence
    Gene expression and gene regulation — bacteria transformed with GFP show green fluorescence only when they are grown in the presence of Antibiotic. Use this experiment to illustrate that not all genes are expressed at the same time and that environmental factors control when gene expression takes place

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    Kit Content

    Materials Provided in Kit

    Storage

    upon Receipt

    Positive Control Plasmid (pUC18-GFP)

    -20°C

    E.coli (Host Cell)

    -20°C

    Ampicillin(100mg/ml)

    -20°C

    0.1M Calcium chloride

    2°C – 8°C

    Luria Bertani(LB)Broth

    RT

    Agar

    RT

     

    Storage Conditions and Product Stability

    All solutions should be kept tightly sealed and stored at above define temperature. These reagents should remain stable for at least 1 year in their unopened containers.

     

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    Items Cat. # Size
    Bacterial Transformation Kit (GFP Based) SCMT010 20 Preps.

     

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    Bacterial Transformation Kit (GFP Based)

    For Transformation and Expression of GFP (green fluorescent protein) gene

    Features
    • Rapid Transformation of Ready to use Clone with GFP Gene
    • No need of cooling centrifuge
    • User friendly protocol for competent cell preparation and transformation
    • Easy screening of transformed cells by phenotypic expression of Green Fluorescent Protein
    Edit Content
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    Items Cat. # Size
    Bacterial Transformation Kit (GFP Based) SCMT010 20 Preps.