SANGER SEQUENCING

"We provide a wide range of Sanger DNA sequencing services".

SLS Research is having the latest version of 3730xl DNA Analyzer has an upgraded solid-state laser with 96-capillary. Like its predecessor, it is the gold standard for high-throughput genetic analysis.

The DNA analyzer is used for DNA fragment analysis applications such as microsatellites, AFLP, and SNP analysis, mutation detection, and traditional DNA sequencing.

Get the highest quality data at a low cost per sample.

Higher optical sensitivity and advanced polymers enable you to obtain higher-quality sequencing data for less Multiple automation features decrease costly human errors Optimized polymers increase your productivity without compromising your results Perform a wide variety of sequencing and fragment analysis applications including resequencing, microsatellite analysis, AFLP, LOH, SSCP, and SNP screening, and SNP validation

SLS is offering this service to find out ribosomal RNA sequence by DNA sanger sequencing corresponding to the rRNA (16S/18S /ITS/26S rRNA). We provide all-inclusive service performing gDNA/mtDNA isolation, amplicon generation, sequencing and consensus. A consensus sequence was used to carry out BLAST with the database of NCBI GenBank. Based on maximum identity score first ten sequences were selected and aligned using multiple alignment software program Clustal W. Distance matrix was generated and the phylogenetic tree was constructed using bioinformatics tools

Hasten DNA Sequencing Services
We provide end to end solutions for your needs like gDNA isolation from tissue to Sequencing and identification services in fast track mode.

DNA / RNA Isolations
We do DNA / RNA isolation’s from the different tissues like Animal, Plant, Insect, Soil etc.
PCR amplification
SLS Team have expertise in standardizing PCR conditions for the target genes.

PURIFICATION OF PCR AMPLICON

We do the PCR Cleanup of the amplified PCR products as mentioned below.

Column Purification
Gel Purification
Exo-SAP

SEQUENCING

SLS is providing high quality results with normal read length of 1000 bp

Free Standard primer
Custom analysis on request
Primer walking

DON’T KNOW THE ENTIRE SEQUENCE OF YOUR PCR OR PLASMID PRODUCT?

Identify unknown regions of a complete plasmid or PCR fragment longer than 1.5 kbp. Our primer walking service allows rapid and precise identification of an entire plasmid or PCR fragment longer than 1.5 Kbp. We offer the best delivery times for your primer walking project.

SLS offers two services under this:

Primer walking single direction
Primer walking bi-direction

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SANGER SEQUENCING

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