SLS’ RNA Tissue Kit is designed for the purification of total, intact RNA from tissue samples, free of any contaminating DNA. The SLS’ RNA Tissue Kit rapidly isolates total RNA from mammalian tissues such as mouse liver, spleen, lung, and heart.

Nucleic acids bind to the surface of the glass fleece in the presence of a chaotropic salt (guanidine HCl). This allows the Silica Column to specifically immobilize nucleic acids (both DNA and RNA) while they are freed of contaminants. For RNA isolation, the binding conditions can be optimized to ensure immobilization of all the RNA.

Capacity: The Spin Columns hold up to 700 μL sample volume.

Sample Material: Solid tissue (e.g., mouse liver, spleen, lung, heart): 5 – 30 mg.

Note: Sample size depends on the method used to prepare the tissue; larger samples (>10 mg) should be processed via rotor-stator homogenization.

Principle

A single reagent which contains a chaotropic salt and detergent lyses the sample and simultaneously inactivates RNases. After cell debris is removed by centrifugation, the lysate is applied to the glass fiber fleece in a Spin column. Under the buffer conditions used in the procedure, nucleic acids bind to the glass fiber fleece, while contaminating substances (salts, proteins, and other cellular contaminants) do not. DNA in the preparation is digested with DNase I directly on the filter. Brief wash-and-spin steps readily remove the digested DNA fragments and other contaminating substances. Once purified, the RNA can be easily eluted in a small volume of low-salt buffer.

Quickly isolate total RNA from a variety of tissues such as mouse liver, spleen, lung, and heart.

Prepare RNA samples in approximately 30 minutes.

Obtain concentrated RNA that is suitable for downstream applications.

Minimize RNA loss with a kit that removes contaminants without precipitation or other handling steps that degrade RNA.

Eliminate the use of hazardous organic compounds such as cesium chloride, phenol, chloroform, and ethidium bromide.
Applications

• RNA-seq
• Quantitative, real-time RT-PCR
• Real-time RT-PCR starting with as little as one cell
• End-point RT-PCR
• Northern, dot, and slot blotting
• Array analysis
• Poly A+ RNA selection