SLS’s T4 DNA Ligase Master Mix facilitates the joining of abutted 5’-phosphate and 3’-hydroxyl termini in duplex DNA through the formation of a phosphodiester bond. It can be used to join DNA fragments with both sticky ends and blunt ends, and to repair nicks in double-stranded DNA with 3′-hydroxyl and 5′-phosphate ends. Anza T4 DNA Ligase is formulated as a 4X concentrated master mix. Ligation can be performed with DNA in water, TE, elution buffer.

Applications:

1. Cloning (blunt-end or cohesive-end ligation)
2.  Adding linkers or adapters to blunt-ended DNA (2).