Restriction Mapping Kit

Restriction mapping is the physical way of mapping a DNA which determine the relative regions of restriction enzyme cleavage sites.

Features

Use to map an unknown segment of DNA by breaking it into pieces and then identifying the locations of breakpoints.

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SLS’ Restriction Mapping Kit, by using this students can perform restriction mapping by completing structural information of phage lambda DNA by using two different restriction enzymes, EcoR I and Hind III. It involves the digestion of phage lambda DNA with a series of restriction enzymes, EcoR I and Hind III and then performing agarose gel electrophoresis method to separate the digested DNA fragments and measure the molecular size of the same.

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Kit Content

Materials Provided in Kit	Storage upon Receipt
Restriction Enzyme: EcoR I	-20°C
Restriction Enzyme: Hind III	-20°C
Lambda DNA (uncut; as a substrate)	-20°C
10X Digestion Assay buffer	-20°C
Lambda DNA Eco/Hind Digest Marker	-20°C
10X Gel loading buffer	-20°C
Nuclease Free Water	-20 °C

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at above define temperature. These reagents should remain stable for at least 1 year in their unopened containers.

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Items	Cat. #	Size
Restriction Mapping Kit	SCMT004	10 Preps.
Restriction Mapping Kit	SCMT004A	20 Preps.

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