Restriction Digestion Kit

To perform restriction digestion of DNA and find out the molecular weight of each fragment of DNA on agarose gel along with a DNA molecular weight marker.

This DNA analysis kit demonstrates the procedures and principles of DNA restriction digestion. Digestion of phage lambda DNA is followed by agarose gel electrophoresis.

Features

Fast digest restriction endonuclease enzymes for rapid and accurate digestion of the DNA Molecule
Use of two different enzymes to digest the sample DNA

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Electrophoretic techniques that distinguish DNA fragments by size are essential in forensics and in the mapping of restriction sites within genes. With the Restriction Digestion and Analysis of Lambda DNA Kit, students use two different restriction enzymes to digest genomic DNA from lambda bacteriophage. By visualizing the effects of two different enzymes on identical samples of double-stranded DNA, students learn that different restriction enzymes recognize and cut different DNA sequences.

With this kit, students are able to:

Use agarose gel electrophoresis to study digestion patterns
Generate standard curves from series of DNA size standards
Analyze migration distances to determine DNA fragment sizes

Students learn the procedures and principles of restriction digestions and DNA gel electrophoresis, including:

Agarose gel casting
Sample loading
Size-based separation of DNA fragments
DNA staining
Graphic analysis

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Kit Content

Materials Provided in Kit	Storage upon Receipt
Restriction Enzyme: EcoR I	-20°C
Restriction Enzyme: Hind III	-20°C
Lambda DNA (uncut; as a substrate)	-20°C
10X Digestion Assay buffer	-20°C
Lambda DNA Eco/Hind Digest Marker	-20°C
10X Gel loading buffer	-20°C
Nuclease Free Water	-20 °C

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at above define temperature. These reagents should remain stable for at least 1 year in their unopened containers.

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Items	Cat. #	Size
Restriction Digestion Kit	SCMT002	10 Preps.
Restriction Digestion Kit	SCMT002A	20 Preps.

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