Multiplex PCR Kit

The kit demonstrates the amplification of desired gene fragments from the template DNA by Polymerase Chain Reaction(PCR).

Features

Rapid and user friendly protocol
Helps the students to understand the concept and methodology of PCR

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Multiplex-PCR is a variant of PCR, which enables simultaneous amplification of many targets of interest in one reaction by using more than one pair of primers. This method has been applied in many areas of DNA testing, including analysis of deletions, mutations, and polymorphisms or quantitative assays and reverse transcriptase PCR. Typically, it is used for genotyping applications where simultaneous analysis of multiple markers is required, detection of pathogens or genetically modified organisms (GMO’s) or for microsatellite analysis.

This kit enables the user to perform multiplex PCR using DNA containing the three loci considered to be amplified using six primers (3 forward and 3 reverse) that result in three bands, 300bp, 400bp and 500bp, respectively. The amplified products are then analysed by running the sample on 2.0% agarose gel along with a marker.

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Kit Content

Materials Provided in Kit	Storage upon Receipt
2X PCR Master Mix	-20°C
Taq DNA Polymerase	-20°C
Template DNA	-20°C
Nuclease Free Water	-20°C
Gel Loading Dye	-20°C
DNA Marker(100 bp Ladder)	-20°C

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at above define temperature. These reagents should remain stable for at least 1 year in their unopened containers.

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Items	Cat. #	Size
Multiplex PCR Teaching Kit	SCMT015	10 Preps.

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