Lambda DNA is digested with Eco RI and HindIII then purified and dissolved in storage buffer. Lambda DNA/EcoRI/HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. The Eco RI/HindIII digest of lambda DNA (cI857ind1 Sam 7) yields 11 fragments suitable for use as molecular weight standards for agarose gel electrophoresis. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.

This marker can be labeled radioactively with T4 Polynucleotide Kinase. Alternatively, it can be labeled radioactively or non-radioactively with Klenow fragment or Klenow Fragment, exo- with the fill-in reaction.