GFP Gene Cloning and Transformation Kit

For cloning and Expression of GFP (green fluorescent protein) gene

Features

Rapid ligation of compatible vector and insert
No need of cooling centrifuge
User friendly protocol for competent cell preparation and transformation
Easy screening of transformed cells by phenotypic expression of Green Fluorescent Protein

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SLS’ GFP Cloning Kits offer engaging and unforgettable lab activities in which students engineer bacteria to express the Green Fluorescent Protein (GFP) and glow green under UV light. These kits enable hands-on learning about the central dogma, gene expression and regulation, genetic engineering, and the biomanufacturing process.

GFP cloning kit enables students to learn the process of gene cloning and the clone confirmation is done by the phenotypic expression of green fluorescent protein (GFP) in E.coli.

Features and Benefits

Hands-on genetic engineering — from handling plasmids to performing heat shock and plating the transformants, students perform the same transformation protocol used by scientists on a daily basis around the world

Visible, dramatic phenotype — bacteria transformed with GFP Clone glow a brilliant fluorescent green under UV light. Once students transform bacteria with GFP Clone, they never forget the central dogma of molecular biology: DNA > RNA > Protein > Trait — Green Fluorescence

Gene expression and gene regulation — bacteria transformed with GFP show green fluorescence only when they are grown in the presence of Antibiotic. Use this experiment to illustrate that not all genes are expressed at the same time and that environmental factors control when gene expression takes place.

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Kit Content

Materials Provided in Kit	Storage upon Receipt
Host Strain	-20°C
Vector	-20°C
Insert	-20°C
T4 DNA Ligase	-20°C
T4 DNA Ligase Assay Buffer	-20°C
Control Plasmid	-20°C
Transformation Solutions	2°C – 8°C
Media Components	RT
Antibiotic	-20°C

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at above define temperature. These reagents should remain stable for at least 1 year in their unopened containers.

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Items	Cat. #	Size
GFP Gene Cloning and Transformation Kit	SCMT007	10 Preps.
GFP Gene Cloning and Transformation Kit	SCMT007A	20 Preps.

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