SLS’ Gel DNA Extraction Kit is designed for the rapid preparation and purification of DNA fragments that have been fractionated on agarose gels. This kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of DNA fragments from gels (up to 400 mg slices) or enzymatic reactions. DNA ranging from 100 bp to 10 kb is purified using a simple and fast bind-wash-elute procedure and an elution volume of 30–50 μl. An integrated pH indicator allows easy determination of the optimal pH for DNA binding to the spin column.

The recovered DNA is free from agarose and other impurities, and is compatible with restriction enzyme digestion, ligation into vectors and sequencing.  The protocol can be completed in 20 minutes.

Principle

SLS’ Gel DNA Extraction Kits contain a silica membrane assembly for binding of DNA in high-salt buffer and elution with low-salt buffer or water. The purification procedure removes primers, nucleotides, enzymes, mineral oil, salts, agarose, ethidium bromide, and other impurities from DNA samples. Silica-membrane technology eliminates the problems and inconvenience associated with loose resins and slurries. Specialized binding buffers are optimized for specific applications and promote selective adsorption of DNA molecules within particular size ranges.

Applications

DNA fragments purified are ready for direct use in all applications, including sequencing, ligation and transformation, restriction digestion, labeling, microinjection, PCR, and in vitro transcription.