DNA Amplification Kit (PCR Teaching Kit)

This kit demonstrates the amplification of desired fragment from the template DNA by Polymerase Chain Reaction(PCR).

Features

Rapid and user friendly protocol
Helps the students to understand the concept and methodology of PCR

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SLS’ DNA Amplification Teaching Kit demonstrates how to set up the PCR reaction and the thermal cycler machine for the amplification of desired gene fragment.

Principle:

Polymerase chain reaction (PCR) is a method widely used to rapidly make millions to billions of copies of a speci?c Region of gene, allowing scientists to take a very small sample of DNA and amplify it to a large enough amount to study in detail.

A basic PCR set-up requires several components including:

A DNA template that contains the DNA target region to amplify

• A DNA polymerase: an enzyme that polymerizes new DNA strands;

• Two DNA primers that are complementary to the 3′ ends of each of the sense and anti-sense strands of the DNA target.

• dNTPs- the building blocks from which the DNA polymerase synthesizes a new DNA strand

• A Buffer Solution providing a suitable chemical environment for optimum activity and stability of the DNA polymerase.

The reaction is commonly carried out in a volume of 10–200 μL in small reaction tubes in a thermal cycler.

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Kit Content

Materials Provided in Kit	Storage upon Receipt
2X PCR Master Mix	-20°C
Taq DNA Polymerase	-20°C
Template DNA	-20°C
Nuclease Free Water	-20°C
Gel Loading Dye	-20°C
DNA Marker(100 bp Ladder)	-20°C

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at above define temperature. These reagents should remain stable for at least 1 year in their unopened containers.

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Items	Cat. #	Size
DNA Amplification Kit (PCR Teaching Kit)	SCMT011	10 Preps.
DNA Amplification Kit (PCR Teaching Kit)	SCMT011A	20 Preps.

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