Agar Agar-Tissue Culture Grade

The major application of Agar Agar is in the field of plant tissue culture. The growing interest in tissue culture as a standard method for the propagation of orchids and other ornamental plants, vegetables, fruits and other agricultural products.

Edit Content

Agar is used extensively in plant biology as it is optionally supplemented with a nutrient and/or vitamin mixture that allows for seedling germination in Petri dishes under sterile conditions.

Agar comes from seaweed or red algae, and is not only used as a gelling agent in the tissue culture process, but also throughout other industries too. This dextrous substance has been utilized in Biotechnology, pharmaceuticals, dentistry, microbiology, and even cosmetics due to the following characteristics:

Thickener
Stabilizer
Gelling agent

Edit Content

Parameters	Values
Clarity	Clear
Moisture	15% max
pH	6.0 – 7.5
Ash	< 3% max
Gel Strength	850 – 950 gm/cm²
Gelling Temperature	36 – 40°C
Melting Temperature	90 -97°C
Swelling Index	> 8 ml
Water Absorption	55 – 67 ml
Gelatin	Passes Test
Extraneous Matter	< 0.5%
Starch	Nil
Acid Insoluble Ash	< 1%
Total Colony Count	< 1000 cfu/gm
Enterobacteriaceae	Absent
Coliform Bacteria	Absent
Salmonella	Absent
E.COLI	Absent
MOLD	Less than 100
YEAST	Less than 100
ARSENIC(AS As)	<3 PPM
HEAVY METALS	<20 PPM
LEAD(AS Pb)	<5 PPM