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    2X PCR Master mix

    2X PCR Master mix

    • Convenient premix format—just add template, primers, and water to the 2X PCR master mix
    • Improved performance—includes an optimized buffer for better amplification of GC- and AT-rich targets
    • High-throughput PCR—use reactions directly in TA cloning, restriction enzyme digestion, or sequencing

     

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    PCR Master Mix is a 2X concentrated solution of dNTPs and all other components required for PCR, except DNA template, primers and Taq DNA Polymerase. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR

     

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    Composition of the PCR Master Mix (2X):

    2X Reaction buffer contains Tris HCl, KCl, 3 mM MgCl2, 0.4 mM of dNTPs (dATP, dCTP, dGTP and dTTP) and other additives.
    PCR Master Mix kit (2X) 25 Rxns. SCMB009
    PCR Master Mix kit (2X) 50 Rxns. SCMB009A
    SafeAmp 2 XPCR Master Mix 40 Rxns. SCMB010
    SafeAmp 2 XPCR Master Mix 160 Rxns. SCMB010
    SafeAmp 2 XPCR Master Mix-HF
    (High Fidelity)
    40 Rxns. SCMB011
    SafeAmp 2 XPCR Master Mix-HF
    (High Fidelity)
    160 Rxns. SCMB011
    SafeAmp 2 X PCR Master Mix-HS 80 Rxns. SCMB012
    SafeAmp 2X SYBR PCR MasterMix-HS 50 Rxns. 50μl SCMB013
    SafeAmp 2X SYBR PCR MasterMix-HS 100 Rxns. of 50μl SCMB013A
    SafeAmp 2X  PCR MasterMix Probe (qPCR) 200 Rxns. SCMB014
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    2X PCR Master mix

    • Convenient premix format—just add template, primers, and water to the 2X PCR master mix
    • Improved performance—includes an optimized buffer for better amplification of GC- and AT-rich targets
    • High-throughput PCR—use reactions directly in TA cloning, restriction enzyme digestion, or sequencing

     

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