gDNA Purification kit ( Bacteria, Yeast, Tissue)
gDNA Purification kit ( Bacteria, Yeast, Tissue)
For isolation of Genomic, Mitochondrial, Bacterial, Parasite or viral DNA
Features
- Isolate genomic DNA from wide range of specimens including cells, bacteria, yeast, tissue, FFPE, saliva, urine and many more
- Rapid, convenient and Reproducible spin column protocol
- Efficiently extract inhibitor-free total genomic DNA
- No organic extraction or alcohol precipitation
- Consistent, high yields
Application
Purified DNA is suitable for sensitive downstream applications including PCR, qPCR, genotyping and sequencing
Introduction
SLS' gDNA Extraction kit(mini) provide user friendly and easy methods for purification of total DNA from small amount of samples for various downstream processing.
This simple protocol which is ideal for simultaneous processing of multiple samples, yield pure DNA ready for direct amplification in few minutes. This protocol requires no phenol/chloroform extraction or alcohol precipitation, and involves very little handling. DNA is eluted in Buffer DE, ready for direct addition to PCR or other enzymatic reactions. Alternatively, it can be safely stored at –20°C for later use.
The purified DNA is free of protein, nucleases, and other contaminants or inhibitors.
Principle
SLS’ gDNA Extraction kit(mini) is designed for rapid purification of total DNA (e.g., genomic, mitochondrial) from small quantity of samples.
Cells are lysed during a short incubation with Proteinase K in the presence of a chaotropic salt, which immediately inactivates all nucleases. Cellular nucleic acids bind selectively to glass fibre membrane in a special centrifuge tube. The nucleic acids remains bound while a series of rapid “wash-and-spin” steps which removes contaminating small molecules. Finally, low salt elution removes the nucleic acids from the glass fibre membrane.
The process does not require precipitation, organic solvent extractions, or extensive handling of the nucleic acids.
For Research Use Only. Not for use in diagnostic procedures.
| Kit Specifications | |
|---|---|
| Maximum Volume of Blood Sample | 200μl |
| Maximum Volume of Bacterial Sample | 2 x 109 cells |
| Capacity of Column Reservoir | 700μl |
| Binding capacity of membrane (ds DNA) | 50μg |
| Elution volume (Maximum) | 200μl |
* Yield will vary depending on the type of sample processed
| Kit Components | ||
|---|---|---|
| Components |
#SCMR006 25 Prep. |
#SCMR006A 50 Prep. |
| Spin Columns with Collection Tubes | 25 Nos. | 50 Nos. |
| Buffer DR | 8 ml | 16 ml |
| Buffer DTL | 8 ml | 16 ml |
| Buffer DL | 8 ml | 16 ml |
| Buffer DW1(Concentrate) | 7 ml | 14 ml |
| Buffer DW2(Concentrate) | 3 ml | 6 ml |
| Buffer DE | 5 ml | 10 ml |
| Proteinase K | 0.5 ml | 1.0 ml |
Storage Conditions and Product Stability
The Proteinase K should be stored at -20°C upon arrival or after reconstitution.
All other solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
| Items | Cat. # | Size |
|---|---|---|
|
Genomic DNA Purification Kit ( Bacteria, Yeast, Tissue) |
SCMR006 | 25 Prep. |
|
Genomic DNA Purification Kit ( Bacteria, Yeast, Tissue) |
SCMR006A | 50 Prep. |
ISO 9001:2015 Registered