SafeAmp PCR Master Mix-High Fidelity
SafeAmp PCR Master Mix-High Fidelity
- Streamlined workflow—simply add template, primers, and water to the 2X master mix then load reactions directly onto gel.
- Ease-of-use—green loading dye tracks migration during electrophoresis.
- High yield—obtain at least 10 times more end product.
- Long PCR—amplify targets up to 15 kb in length.
- Easy integration with downstream application—perform restriction digests directly in the PCR buffer or use in TA cloning.
SafeAmp PCR Master Mix-HF, a dye-added (green) 2X PCR master mix for high-yield PCR. Reactions performed with this master mix can be loaded directly onto an agarose gel for electrophoresis.
SafeAmp PCR Master Mix-HF is a 2X premix composed of an optimized buffer, PCR enzyme, dNTP mixture, gel loading dye (green), and a density reagent. The green dye separates into blue and yellow dye fronts when the PCR product is run on an agarose gel. The master mix format greatly simplifies workflows and sample handling; add primers, template, and water and begin PCR. SafeAmp Master Mix also allows amplification of long products, it is possible to amplify 15 kb genomic DNA fragments.
Kit Content:
- 1 ml PCR 2X MasterMix (for 40 Preparations)
- 1 ml Sterile PCR Grade Water
Storage:
-20? for long-term storage. 4? for short-term storage (up to 3 months).If used frequently, store at 4?; repeated freezing and thawing will decrease its activity. Gently mix well before use and centrifuge briefly.
PCR Products: Most PCR products amplified with this product have one A added at 3’-termini. Thus, the PCR product can be used directly for cloning into a T-vector. Additionally, it is possible to clone the product in blunt-end vectors after blunting and phosphorylation
Dye Migration during Electrophoresis:
If 5 μl of the PCR sample is used for electrophoresis on a 1% Agarose gel, the blue dye front migrates near 3 - 5 kb and the yellow dye front migrates below 50 bp. The absorption maxima for the dyes are ~ 260 nm and 420 nm, respectively. The dyes may be removed by excising and purifying DNA from the gel or extracting DNA with PCR Clean-up Kit, if necessary
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