Enzyme

Cut site and sequence

Overhang

Optimal reaction temperature

Thermal inactivation

Pack Size

Cat. No.

EcoRI

G^AATTC

CTTAA^G

5’ protruding ends

37° C

80°C, 5min

2000 U

5000U

SCRM001
SCRM001L

HindIII

GTY^RAC

CAR^YTG

5’ protruding ends

37° C

65°C, 5min

2000 U

5000U

SCRM002
SCRM002L

BamHI

G^GATCC

CCTAG^G

5’ protruding ends

37° C

80°C, 5min

2000 U

5000U

SCRM003
SCRM003L

Eco RV

GAT^ATC

CTA^TAG

5’ protruding ends

37° C

No
(column purification)

2000 U

5000U

SCRM004
SCRM004L

XbaI

T^CTAGA

AGATC^T

5’ protruding ends

37° C

65°C, 20 min

500 U

SCRM005

XhoI

C^TCGAG

GAGCT^C

5’ protruding ends

37° C

80°C, 5min

500 U

SCRM006

KpnI

GGTAC^C

C^CATGG

3’ protruding ends

37° C

80°C, 5min

2000 U

SCRM007

NcoI

C^CATGG

GGTAC^C

5’ protruding ends

37° C

65°C, 15min

500 U

SCRM008

NotI

GC^GGCCGC

CGCCGG^CG

5’ protruding ends

37° C

80°C, 5min

1000 U

SCRM009

PstI

CTGCA^G

G^ACGTC

3’ protruding ends

37° C

No
(column purification)

1000 U

SCRM010

PvuI

CGAT^CG

GC^TAGC

3’ protruding ends

37° C

80°C, 5min

500 U

SCRM011

PvuII

CAG^CTG

GTC^GAC

Blunt ends

37° C

No
(column purification)

1000 U

SCRM012

SmaI

CCC^GGG

GGG^CCC

Blunt ends

37° C

65°C, 5min

1000 U

SCRM013

TaqI

T^CGA

AGC^T

5’ protruding ends

65° C

No
(column purification)

500 U

SCRM014

SspI

AAT^ATT

TTA^TAA

Blunt ends

37° C

65°C, 5min

500 U

SCRM015

SalI

G^TCGAC

CAGCT^G

5’ protruding ends

37° C

65°C, 10 min

500 U

SCRM016