Multiplex-PCR is a variant of PCR, which enables simultaneous amplification of many targets of interest in one reaction by using more than one pair of primers. This method has been applied in many areas of DNA testing, including analysis of deletions, mutations, and polymorphisms or quantitative assays and reverse transcriptase PCR. Typically, it is used for genotyping applications where simultaneous analysis of multiple markers is required, detection of pathogens or genetically modified organisms (GMO’s) or for microsatellite analysis.

This kit enables the user to perform multiplex PCR using DNA containing the three loci considered to be amplified using six primers (3 forward and 3 reverse) that result in three bands, 300bp, 400bp and 500bp, respectively. The amplified products are then analysed by running the sample on 2.0% agarose gel along with a marker.