SLS’ GFP Cloning Kits offer engaging and unforgettable lab activities in which students engineer bacteria to express the Green Fluorescent Protein (GFP) and glow green under UV light. These kits enable hands-on learning about the central dogma, gene expression and regulation, genetic engineering, and the biomanufacturing process.

GFP cloning kit enables students to learn the process of gene cloning and the clone confirmation is done by the phenotypic expression of green fluorescent protein (GFP) in E.coli.

Features and Benefits

Hands-on genetic engineering — from handling plasmids to performing heat shock and plating the transformants, students perform the same transformation protocol used by scientists on a daily basis around the world

Visible, dramatic phenotype — bacteria transformed with GFP Clone glow a brilliant fluorescent green under UV light. Once students transform bacteria with GFP Clone, they never forget the central dogma of molecular biology: DNA > RNA > Protein > Trait — Green Fluorescence

Gene expression and gene regulation — bacteria transformed with GFP show green fluorescence only when they are grown in the presence of Antibiotic. Use this experiment to illustrate that not all genes are expressed at the same time and that environmental factors control when gene expression takes place.