DNA Ligation Kit
DNA Ligation Kit
To perform ligation of two DNA molecules by using the enzyme, DNA ligase.
This DNA Ligation kit can Ligate both blunt and cohesive ends using same buffer.
Features
- Fast—ligation of either sticky- or blunt-end DNA in few minutes
- No additional purification steps required prior to transformation.
- Convenient—reaction mixture can be used directly for bacterial transformation
SLS’ Rapid DNA Ligation Kit enables fast sticky-end or blunt-end DNA ligation in minutes at room temperature. The kit contains T4 DNA ligase and a specially-formulated 10 X rapid ligation buffer optimized for fast and efficient DNA ligation. Fast ligation efficiency is equal to that obtained with T4 DNA ligase in a standard 1 hour ligation. The ligation reaction mixture can be used directly for bacterial transformation with the SLS Bacterial Transformation Kit or with other conventional transformation procedures.
Applications
• Routine cloning experiments
• Blunt-end cloning
• Library construction
• TA cloning
Kit Content
|
Materials Provided in Kit |
Storage upon Receipt |
|
λ / EcoR I Digest |
-20°C |
|
10X Ligase Assay Buffer |
-20°C |
|
T4 DNA Ligase |
-20°C |
|
10X Gel loading buffer |
-20°C |
|
Nuclease Free Water |
-20 °C |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at above define temperature. These reagents should remain stable for at least 1 year in their unopened containers.
| Items | Cat. # | Size |
|---|---|---|
| DNA Ligation Kit | SCMT003 | 10 Preps. |
| DNA Ligation Kit | SCMT003A | 20 Preps. |
ISO 9001:2015 Registered