DNA Double Digestion Kit
DNA Double Digestion Kit
To perform double digestion of DNA and find out the molecular weight of each fragment of DNA on agarose gel along with a DNA molecular weight marker.
This DNA analysis kit demonstrates the procedures and principles of DNA Double digestion. Digestion of phage lambda DNA is followed by agarose gel electrophoresis.
Features
- Fast digest restriction endonuclease enzymes for rapid and accurate digestion of the DNA Molecule
- Use of two different enzymes to digest the sample DNA
Electrophoretic techniques that distinguish DNA fragments by size are essential in forensics and in the mapping of restriction sites within genes. With the Restriction Digestion and Analysis of Lambda DNA Kit, students use two different restriction enzymes to digest genomic DNA from lambda bacteriophage. By visualizing the effects of two different enzymes on identical samples of double-stranded DNA, students learn that different restriction enzymes recognize and cut different DNA sequences.
Kit Content
|
Materials Provided in Kit |
Storage upon Receipt |
|
Restriction Enzyme: EcoR I |
-20°C |
|
Restriction Enzyme: Hind III |
-20°C |
|
Lambda DNA (uncut; as a substrate) |
-20°C |
|
10X Digestion Assay buffer |
-20°C |
|
Lambda DNA Eco/Hind Digest Marker |
-20°C |
|
10X Gel loading buffer |
-20°C |
|
Nuclease Free Water |
-20 °C |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at above define temperature. These reagents should remain stable for at least 1 year in their unopened containers.
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Items |
Cat. # |
Size |
|---|---|---|
|
Restriction Digestion Kit |
SCMT006 |
10 Preps. |
|
Restriction Digestion Kit |
SCMT006A |
20 Preps. |
ISO 9001:2015 Registered