Agarose Gel Electrophoresis Kit
Agarose Gel Electrophoresis Kit
For separation of DNA molecules on agarose gel and determining their size with the DNA ladder.
Features
- Easy and user friendly procedure
- Determining the size of the sample DNA bands with DNA Ladder
Agarose gel electrophoresis is a procedure used to separate DNA fragments based on their molecular weight and is an intrinsic part of almost all routine experiments carried out in molecular biology.
The kit teaches the three basic steps involved in this technique i.e. preparation of agarose gel, electrophoresis of the DNA fragments, visualization of DNA fragments.
SLS’ Agarose gel electrophoresis teaching kit enables the students to visualize the DNA fragments based on their molecular weight on agarose gel.
Principle:
Agarose gel electrophoresis; a method for separation of DNA and RNA molecules based on their molecular size and charge. The negatively charged nucleic acids migrate through an agarose gel matrix under the influence of an electric field. Smaller molecules move faster and migrate farther than the larger ones. The position of DNA in the agarose gel is visualised by staining with low concentration of fluorescent intercalating dyes, such as Ethidium bromide.
Kit Content
|
Materials Provided |
Storage upon Receipt |
|
Genomic DNA (as a Reference Sample) |
-20°C |
|
DNA Ladder |
-20°C |
|
6X Gel Loading Buffer |
-20°C |
|
10 X TAE |
RT |
|
Agarose, Low EEO |
RT |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at the temperature mentioned above. These reagents should remain stable for at least 1 year in their unopened containers at their storage temperature.
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Items |
Cat. # |
Size |
|---|---|---|
|
Agarose Gel Electrophoresis Kit |
SCMT001 |
10 Prep. |
ISO 9001:2015 Registered