2X PCR Master mix
2X PCR Master mix
- Convenient premix format—just add template, primers, and water to the 2X PCR master mix
- Improved performance—includes an optimized buffer for better amplification of GC- and AT-rich targets
- High-throughput PCR—use reactions directly in TA cloning, restriction enzyme digestion, or sequencing
PCR Master Mix is a 2X concentrated solution of dNTPs and all other components required for PCR, except DNA template, primers and Taq DNA Polymerase. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR
Composition of the PCR Master Mix (2X):
2X Reaction buffer contains Tris HCl, KCl, 3 mM MgCl2, 0.4 mM of dNTPs (dATP, dCTP, dGTP and dTTP) and other additives.
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