Plant gDNA Purification Kit (Plant, Fungi)

For extraction of total cellular DNA from plant cells and tissues or fungi, or genomic DNA from plant cells, tissues and seeds

Rapid and simple procedure
No organic extraction, no ethanol precipitation
Excellent quality and yield of DNA
Process a broad spectrum of plant species and filamentous fungi
Highly efficient lysis and release of DNA from tough plant materials and associated plant pathogens

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This kit provides a rapid method for the isolation and purification of total DNA from a wide range of plant and fungal species. Total DNA, including genomic DNA, mitochondrial DNA and chloroplast DNA can be purified from fresh or frozen plant tissues, plant cells or fungi samples using this kit. Purified DNA samples can be used for the detection of viral pathogens, as viral DNA is isolated with the plant/fungi DNA. This Kits provide fast and easy silica-based DNA isolation from plant samples in spin column format. Typical yields are 3–200 μg of high-quality DNA, depending on the samples used (e.g., wheat, maize, Arabidopsis, tomato, tobacco) and binding capacity of the silica membrane.

The purified DNA is of the highest integrity, and can be used in a number of downstream applications including PCR, qPCR, SNP, Southern blotting and sequencing.

Applications

This Kit provide purification of ready-to-use DNA from plant samples, including plant cells, plant tissues and fungi.

For Research Use Only. Not for use in diagnostic procedures.

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Kit Specifications – Spin Column
Column Binding Capacity	Up to 50 μg
Maximum Column Loading Volume	650 μL
Elution volume	50–200 µl
Processing	Manual
Technology	Silica technology
Time per run or per prep	<1 hour
Maximum Amount of Starting Material:
Plant Tissues	Up to 100 mg
Fungi (wet weight)	100 mg
Average Yields*
50 mg Tomato Leaves	18 µg
50 mg Grape Leaves	10 µg
50 mg Peach Leaves	10 µg
50 mg Plum Leaves	10 µg
50 mg Pine Needles	5 µg
Botrytis cinerea (50 mg wet weight)	1.5 µg
Fusarium sp. (50 mg wet weight)	2 µg
Aspergillus niger (50 mg wet weight)	4 µg

* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.

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Items	Cat. #	Size
Plant gDNA Purification Kit (Plant & Fungi)	SCMR007	25 Prep.
Plant gDNA Purification Kit (Plant & Fungi)	SCMR007A	50 Prep.

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Plant gDNA Purification Kit (Plant, Fungi)

For extraction of total cellular DNA from plant cells and tissues or fungi, or genomic DNA from plant cells, tissues and seeds

Rapid and simple procedure
No organic extraction, no ethanol precipitation
Excellent quality and yield of DNA
Process a broad spectrum of plant species and filamentous fungi
Highly efficient lysis and release of DNA from tough plant materials and associated plant pathogens