RAPD Teaching KIT

This Kit helps to understand the concept of DNA fingerprinting by Random Amplification of Polymorphic DNA (RAPD) in context of bacterial strain identification.

The kit demonstrates RAPD technique in context to strain identification.

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DNA Fingerprinting is a well known technique for establishing authenticity. Apart from its forensic applications, it has also evolved as a simple, yet elegant technique in solving paternity disputes, confirming quality parameters in plant biotech industries, characterizing microorganisms and other applications in medical sciences.

RAPD (Random Amplified Polymorphic DNA) is a PCR based technique that makes use of random primers that bind to a number of partially or perfectly complimentary sequences at unknown locations in the genome of an organism and produce specific bands (DNA fingerprints) that are unique. The principle is that, the single, short oligonucleotide primer, which binds to many different loci, is used to amplify random sequences from a complex DNA template. This means that the amplified fragment generated by PCR depends on the length and size of both the primer and the target genome.

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Kit Content

Materials Provided in Kit	Storage upon Receipt
2X PCR Master Mix (contains Random Primers)	-20°C
Taq DNA Polymerase	-20°C
3 samples of Template DNA (as Test Samples)	-20°C
Reference Genomic DNA	-20°C
Nuclease Free Water	-20°C
Gel Loading Dye	-20°C
DNA Marker(100 bp Ladder)	-20°C

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at above define temperature. These reagents should remain stable for at least 1 year in their unopened containers.

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Items	Cat. #	Size
RAPD Teaching Kit	SCMT012	10 Preps.

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